Substrate recognition and proton coupling by a bacterial member of solute carrier family 17.

ABSTRACT: The solute carrier 17 family transports diverse organic anions using two distinct modes of coupling to a source of energy. Transporters that package glutamate and nucleotide into secretory vesicles for regulated release by exocytosis are driven by membrane potential but subject to allosteric regulation by H⁺ and Cl^-. Other solute carrier 17 members including the lysosomal sialic acid exporter couple the flux of organic anion to cotransport of H⁺. To begin to understand how similar proteins can perform such different functions, we have studied Escherichia coli DgoT, a H⁺/galactonate cotransporter. A recent structure of DgoT showed many residues contacting D-galactonate, and we now find that they do not tolerate even conservative substitutions. In

SUBMITTER: Batarni S 

PROVIDER: S-EPMC10149257 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature