Analysis of monoclonal immunoglobulins from bone marrow plasma cells using immunopurification and LC-MS.

ABSTRACT:

Introduction

Clonal plasma cells secrete immunoglobulins, each with the exact same amino acid sequence, that are referred to as monoclonal immunoglobulins. The monoclonal heavy chain and light chain secreted from clonal plasma cells have the same molecular mass prior to the addition of post-translational modifications (PTMs) since their amino acid sequences are the same.

Objective

To examine the molecular masses of monoclonal light chains and heavy chains isolated directly from the cytoplasm of bone marrow (BM) plasma cells and compare them to the serum derived monoclonal heavy and light chains.

Methods

Using immunopurification and LC-MS we compared the molecular masses of immunoglobulins immunopurified from a patient's serum to those immunopurified from the cytoplasm

SUBMITTER: Barnidge DR 

PROVIDER: S-EPMC10149385 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature