Dataset Information

Polymorphisms in the MBL2 gene are associated with the plasma levels of MBL and the cytokines IL-6 and TNF-α in severe COVID-19.

ABSTRACT:

Introduction

Mannose-binding lectin (MBL) promotes opsonization, favoring phagocytosis and activation of the complement system in response to different microorganisms, and may influence the synthesis of inflammatory cytokines. This study investigated the association of MBL2 gene polymorphisms with the plasma levels of MBL and inflammatory cytokines in COVID-19.

Methods

Blood samples from 385 individuals (208 with acute COVID-19 and 117 post-COVID-19) were subjected to real-time PCR genotyping. Plasma measurements of MBL and cytokines were performed by enzyme-linked immunosorbent assay and flow cytometry, respectively.

Results

The frequencies of the polymorphic MBL2 genotype (OO) and allele (O) were higher in patients with severe COVID-19 (p< 0.05). The polymorphic genotypes (AO and OO) were associated with lower MBL levels (p< 0.05). IL-6 and TNF-α were higher in patients with low MBL and severe COVID-19 (p< 0.05). No association of polymorphisms, MBL levels, or cytokine levels with long COVID was observed.

Discussion

The results suggest that, besides MBL2 polymorphisms promoting a reduction in MBL levels and therefore in its function, they may also contribute to the development of a more intense inflammatory process responsible for the severity of COVID-19.

SUBMITTER: Queiroz MAF

PROVIDER: S-EPMC10149935 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature

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Publications

Polymorphisms in the <i>MBL2</i> gene are associated with the plasma levels of MBL and the cytokines IL-6 and TNF-α in severe COVID-19.

Queiroz Maria Alice Freitas MAF Santiago Angélica Menezes AM Brito Wandrey Roberto Dos Santos WRDS Pereira Keise Adrielle Santos KAS de Brito William Botelho WB Torres Maria Karoliny da Silva MKDS Lopes Jeferson da Costa JDC Dos Santos Erika Ferreira EF da Costa Flávia Póvoa FP de Sarges Kevin Matheus Lima KML Cantanhede Marcos Henrique Damasceno MHD de Brito Mioni Thieli Figueiredo Magalhães MTFM da Silva Andréa Luciana Soares ALS Leite Mauro de Meira MM Viana Maria de Nazaré do Socorro de Almeida MNDSA Rodrigues Fabíola Brasil Barbosa FBB da Silva Rosilene R Viana Giselle Maria Rachid GMR Chaves Tânia do Socorro Souza TDSS Veríssimo Adriana de Oliveira Lameira AOL Carvalho Mayara da Silva MDS Henriques Daniele Freitas DF Dos Santos Carla Pinheiro CP Nunes Juliana Abreu Lima JAL Costa Iran Barros IB Amoras Ednelza da Silva Graça EDSG Lima Sandra Souza SS Cayres-Vallinoto Izaura Maria Vieira IMV Brasil-Costa Igor I Quaresma Juarez Antônio Simões JAS Falcão Luiz Fábio Magno LFM Dos Santos Eduardo José Melo EJM Vallinoto Antonio Carlos Rosário ACR

Frontiers in immunology 20230417

<h4>Introduction</h4>Mannose-binding lectin (MBL) promotes opsonization, favoring phagocytosis and activation of the complement system in response to different microorganisms, and may influence the synthesis of inflammatory cytokines. This study investigated the association of MBL2 gene polymorphisms with the plasma levels of MBL and inflammatory cytokines in COVID-19.<h4>Methods</h4>Blood samples from 385 individuals (208 with acute COVID-19 and 117 post-COVID-19) were subjected to real-time PC ...[more]

PMID: 37138871

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