RNA-Seq of untreated wastewater to assess COVID-19 and emerging and endemic viruses for public health surveillance.

ABSTRACT:

Background

The COVID-19 pandemic showcased the power of genomic sequencing to tackle the emergence and spread of infectious diseases. However, metagenomic sequencing of total microbial RNAs in wastewater has the potential to assess multiple infectious diseases simultaneously and has yet to be explored.

Methods

A retrospective RNA-Seq epidemiological survey of 140 untreated composite wastewater samples was performed across urban (n = 112) and rural (n = 28) areas of Nagpur, Central India. Composite wastewater samples were prepared by pooling 422 individual grab samples collected prospectively from sewer lines of urban municipality zones and open drains of rural areas from 3rd February to 3rd April 2021, during the second COVID-19 wave in India. Samples were pre-processed and

SUBMITTER: Stockdale SR 

PROVIDER: S-EPMC10150210 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature