Effects of Nucleic Acid Structural Heterogeneity on the Electrochemistry of Tethered Redox Molecules.

ABSTRACT: The cation condensation-induced collapse of electrode-bound nucleic acids and the resulting change in the electrochemical signal is a useful tool to predict the structure and redox probe location of heterogeneous structures of surface-tethered DNA probes─a common architecture employed in the development of electrochemical sensors. In this paper, we measure the faradaic current of an appended redox molecule at the 3' position of the nucleic acid using cyclic voltammetry before and after nucleic acid collapse for various nucleic acid architectures and heterogeneous mixtures on the same electrode surface. The voltammetric peak current change with collapse correlates with the proximity of the redox molecules from the surface. For stem-loop probes, the terminal methylene blue is initially held

SUBMITTER: Sykes KS 

PROVIDER: S-EPMC10150402 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature