M⁶A readers ECT2/ECT3/ECT4 enhance mRNA stability through direct recruitment of the poly(A) binding proteins in Arabidopsis.

ABSTRACT:

Background

RNA N⁶-methyladenosine (m⁶A) modification is critical for plant growth and crop yield. m⁶A reader proteins can recognize m⁶A modifications to facilitate the functions of m⁶A in gene regulation. ECT2, ECT3, and ECT4 are m⁶A readers that are known to redundantly regulate trichome branching and leaf growth, but their molecular functions remain unclear.

Results

Here, we show that ECT2, ECT3, and ECT4 directly interact with each other in the cytoplasm and perform genetically redundant functions in abscisic acid (ABA) response regulation during seed germination and post-germination growth. We reveal that ECT2/ECT3/ECT4 promote the stabilization of their targeted m⁶A-modified mRNAs, but have no

SUBMITTER: Song P 

PROVIDER: S-EPMC10150487 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature