Anti-inflammatory and antioxidative effects of gallic acid on experimental dry eye: in vitro and in vivo studies.

ABSTRACT:

Background

To investigate the anti-inflammatory and antioxidative effects of gallic acid (GA) on human corneal epithelial cells (HCECs) and RAW264.7 macrophages as well as its therapeutic effects in an experimental dry eye (EDE) mouse model.

Methods

A cell counting kit-8 (CCK-8) assay was used to test the cytotoxicity of GA. The effect of GA on cell migration was evaluated using a scratch wound healing assay. The anti-inflammatory and antioxidative effects of GA in vitro were tested using a hypertonic model (HCECs) and an inflammatory model (RAW264.7 cells). The in vivo biocompatibility of GA was detected by irritation tests in rabbits, whereas the preventive and therapeutic effect of GA in vivo was evaluated using a mouse model of EDE.

Results

In the range of 0-100 μ

SUBMITTER: Li K 

PROVIDER: S-EPMC10150500 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature