An improved method for preparing stained ground teeth sections.

ABSTRACT:

Objective

In oral histopathology teaching and research, there is a need for high-quality undemineralized tooth sections that are easy to handle, have controlled thickness, allow the observation of intact microstructures, and can be preserved for long periods of time.

Methods

Teeth were collected under non-demineralizing conditions. Tooth sections (15-25 µm) were prepared using a diamond knife, then randomly divided into three groups: (1) stained with rosin, (2) stained with hematoxylin and eosin, or (3) not stained. The prepared tooth sections were evaluated by microscopy for clarity and microstructure visibility.

Results

The use of a diamond knife in the sectioning and grinding process yielded high-quality ground sections of teeth. Rosin-stained ground sections allow

SUBMITTER: Qin Q 

PROVIDER: S-EPMC10150715 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature