Identification and validation of reference genes for normalization of gene expression analysis using qRT-PCR in <i>Megalurothrips usitatus</i> (thysanoptera: thripidae).

ABSTRACT: Introduction: Gene expression analysis by reverse transcription quantitative polymerase chain reaction (qRT-PCR) has been widely used in research including insects. The selection of appropriate reference genes is the key to obtaining accurate and reliable results from qRT-PCR. However, studies on the expression stability of reference genes in Megalurothrips usitatus remain lacking. Methods: In this study, qRT-PCR was used to analyze the expression stability of candidate reference genes in M. usitatus. The expression levels of six candidate reference gene transcription of M. usitatus were analyzed. GeNorm, NormFinder, BestKeeper, and ΔCt were used to analyze the expression stability of M. usitatus treated with biological factors (developmental perio

SUBMITTER: Hou Q 

PROVIDER: S-EPMC10151585 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature