Macrophage-derived exosome promotes regulatory T cell differentiation in malignant pleural effusion.

ABSTRACT:

Introduction

Tumor-associated macrophages are one of the key components of the tumor microenvironment. The immunomodulatory activity and function of macrophages in malignant pleural effusion (MPE), a special tumor metastasis microenvironment, have not been clearly defined.

Methods

MPE-based single-cell RNA sequencing data was used to characterize macrophages. Subsequently, the regulatory effect of macrophages and their secreted exosomes on T cells was verified by experiments. Next, miRNA microarray was used to analyze differentially expressed miRNAs in MPE and benign pleural effusion, and data from The Cancer Genome Atlas (TCGA) was used to evaluate the correlation between miRNAs and patient survival.

Results

Single-cell RNA sequencing data showed macrophages were mai

SUBMITTER: Shao MM 

PROVIDER: S-EPMC10151820 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature