YY1 complex in M2 macrophage promotes prostate cancer progression by upregulating IL-6.

ABSTRACT:

Background

Tumor-associated macrophages are mainly polarized into the M2 phenotype, remodeling the tumor microenvironment and promoting tumor progression by secreting various cytokines.

Methods

Tissue microarray consisting of prostate cancer (PCa), normal prostate, and lymph node metastatic samples from patients with PCa were stained with Yin Yang 1 (YY1) and CD163. Transgenic mice overexpressing YY1 were constructed to observe PCa tumorigenesis. Furthermore, in vivo and in vitro experiments, including CRISPR-Cas9 knock-out, RNA sequencing, chromatin immunoprecipitation (ChIP) sequencing, and liquid-liquid phase separation (LLPS) assays, were performed to investigate the role and mechanism of YY1 in M2 macrophages and PCa tumor microenvironment.

Results

YY1 was highly

SUBMITTER: Chen S 

PROVIDER: S-EPMC10152059 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature