In-depth tissue profiling using multiplexed immunohistochemical consecutive staining on single slide.

ABSTRACT: Despite remarkable recent achievements of immunotherapy strategies in cancer treatment, clinical responses remain limited to subsets of patients. Predictive markers of disease course and response to immunotherapy are urgently needed. Recent results have revealed the potential predictive value of immune cell phenotype and spatial distribution at the tumor site, prompting the need for multidimensional immunohistochemical analyses of tumor tissues. To address this need, we developed a sample-sparing, highly multiplexed immunohistochemistry technique based on iterative cycles of tagging, image scanning, and destaining of chromogenic substrate on a single slide. This assay, in combination with a newly developed automated digital landscaping solution, democratizes access to high-dimensional immu

SUBMITTER: Remark R 

PROVIDER: S-EPMC10152404 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature