Signal Amplification in Electrochemical DNA Biosensors Using Target-Capturing DNA Origami Tiles.

ABSTRACT: Electrochemical DNA (e-DNA) biosensors are feasible tools for disease monitoring, with their ability to translate hybridization events between a desired nucleic acid target and a functionalized transducer, into recordable electrical signals. Such an approach provides a powerful method of sample analysis, with a strong potential to generate a rapid time to result in response to low analyte concentrations. Here, we report a strategy for the amplification of electrochemical signals associated with DNA hybridization, by harnessing the programmability of the DNA origami method to construct a sandwich assay to boost charge transfer resistance (R_CT) associated with target detection. This allowed for an improvement in the sensor limit of detection by two orders of magnitude compa

SUBMITTER: Williamson P 

PROVIDER: S-EPMC10152479 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature