Assessment of glycosaminoglycan content in bone using Raman spectroscopy.

ABSTRACT: Glycosaminoglycans (GAGs) are responsible for preserving bone tissue toughness as well as regulating collagen formation and mineralization in the extracellular matrix. However, current methods for characterization of GAGs in bone are destructive, thus unable to capture in situ changes or differences in GAGs between experimental groups. As an alternative, Raman spectroscopy is a non-destructive method and can detect concurrent changes in GAGs and other bone constituents. In this study, we hypothesized that the two most prominent Raman peaks of sulfated GAGs (at ~1066 cm^-1 and at ~1378 cm^-1) could be used to detect differences in GAGs content of bone. To test this hypothesis, three experimental models were utilized: an in vitro model (enzymatic removal of GAGs from huma

SUBMITTER: Heath S 

PROVIDER: S-EPMC10152494 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature