The Murine Oral Metatranscriptome Reveals Microbial and Host Signatures of Periodontal Disease.

ABSTRACT: Periodontal disease is accompanied by alterations to cellular profiles and biological activities of both the subgingival microbiome and host tissues. Although significant progress has been made in describing the molecular basis of the homeostatic balance of host-commensal microbe interactions in health compared to the destructive imbalance in disease, particularly with respect to immune and inflammatory systems, few studies have attempted a comprehensive analysis in diverse host models. Here, we describe the development and application of a metatranscriptomic approach to analysis of host-microbe gene transcription in a murine periodontal disease model, based on oral gavage infection using Porphyromonas gingivalis in C57BL6/J mice. We generated 24 metatranscriptomic libraries from in

SUBMITTER: Joseph S 

PROVIDER: S-EPMC10152569 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature