Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat production.

ABSTRACT: Cultured meat is a promising technology that faces substantial cost barriers which are currently driven largely by the price of media components. Growth factors such as fibroblast growth factor 2 (FGF2) drive the cost of serum-free media for relevant cells including muscle satellite cells. Here, we engineered immortalized bovine satellite cells (iBSCs) for inducible expression of FGF2 and/or mutated Ras^G12V in order to overcome media growth factor requirements through autocrine signaling. Engineered cells were able to proliferate over multiple passages in FGF2-free medium, thereby eliminating the need for this costly component. Additionally, cells maintained their myogenicity, albeit with reduced differentiation capacity. Ultimately, this offers a proof-of-principle for lower-co

SUBMITTER: Stout AJ 

PROVIDER: S-EPMC10153192 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature