DNA-m6A calling and integrated long-read epigenetic and genetic analysis with fibertools.

ABSTRACT: Long-read DNA sequencing has recently emerged as a powerful tool for studying both genetic and epigenetic architectures at single-molecule and single-nucleotide resolution. Long-read epigenetic studies encompass both the direct identification of native cytosine methylation as well as the identification of exogenously placed DNA N⁶-methyladenine (DNA-m6A). However, detecting DNA-m6A modifications using single-molecule sequencing, as well as co-processing single-molecule genetic and epigenetic architectures, is limited by computational demands and a lack of supporting tools. Here, we introduce fibertools, a state-of-the-art toolkit that features a semi-supervised convolutional neural network for fast and accurate identification of m6A-marked bases using PacBio

SUBMITTER: Jha A 

PROVIDER: S-EPMC10153250 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature