Single-cell DNA Methylome and 3D Multi-omic Atlas of the Adult Mouse Brain.

ABSTRACT: Cytosine DNA methylation is essential in brain development and has been implicated in various neurological disorders. A comprehensive understanding of DNA methylation diversity across the entire brain in the context of the brain's 3D spatial organization is essential for building a complete molecular atlas of brain cell types and understanding their gene regulatory landscapes. To this end, we employed optimized single-nucleus methylome (snmC-seq3) and multi-omic (snm3C-seq¹) sequencing technologies to generate 301,626 methylomes and 176,003 chromatin conformation/methylome joint profiles from 117 dissected regions throughout the adult mouse brain. Using iterative clustering and integrating with companion whole-brain transcriptome and chromatin accessibility datasets, we construc

SUBMITTER: Liu H 

PROVIDER: S-EPMC10153407 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature