Dynamics of monocyte-derived macrophage diversity in experimental myocardial infarction.

ABSTRACT:

Aims

Macrophages have a critical and dual role in post-ischaemic cardiac repair, as they can foster both tissue healing and damage. Multiple subsets of tissue resident and monocyte-derived macrophages coexist in the infarcted heart, but their precise identity, temporal dynamics, and the mechanisms regulating their acquisition of discrete states are not fully understood. To address this, we used multi-modal single-cell immune profiling, combined with targeted cell depletion and macrophage fate mapping, to precisely map monocyte/macrophage transitions after experimental myocardial infarction.

Methods and results

We performed single-cell transcriptomic and cell-surface marker profiling of circulating and cardiac immune cells in mice challenged with acute myocardial infarction,

SUBMITTER: Rizzo G 

PROVIDER: S-EPMC10153424 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature