Chikungunya virus infection in Aedes aegypti is modulated by L-cysteine, taurine, hypotaurine and glutathione metabolism.

ABSTRACT:

Background

Blood meal and infections cause redox imbalance and oxidative damage in mosquitoes which triggers the mosquito's system to produce antioxidants in response to increased oxidative stress. Important pathways activated owing to redox imbalance include taurine, hypotaurine and glutathione metabolism. The present study was undertaken to evaluate the role of these pathways during chikungunya virus (CHIKV) infection in Aedes aegypti mosquitoes.

Methodology

Using a dietary L-cysteine supplement system, we upregulated these pathways and evaluated oxidative damage and oxidative stress response upon CHIKV infection using protein carbonylation and GST assays. Further, using a dsRNA based approach, we silenced some of the genes involved in synthesis and transport of taurine an

SUBMITTER: Kumar A 

PROVIDER: S-EPMC10153688 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature