Profile of loiasis infection through clinical and laboratory diagnostics: the importance of biomarkers.

ABSTRACT:

Background

Detection of Loa loa microfilariae in peripheral blood is insensitive given only 30% of individuals are microfilaraemic while 70% are amicrofilaraemic with a variety of clinical signs. Biomarkers may improve the diagnosis of loiasis.

Methods

A total of 545 individuals exposed to L. loa were analysed using clinical data collected through a questionnaire (requesting information on eye worm, Calabar swelling, pruritis) and detection of microfilariae, immunoglobulin G4 (IgG4), DNA and antigens using microscopy, enzyme-linked immunosorbent assay (ELISA), quantitative polymerase chain reaction (qPCR) and Western blot, respectively.

Results

The results revealed that the rates of detection of L. loa microfilariae in the blood, of DNA by qPCR, of IgG4 by ELISA and o

SUBMITTER: Dieki R 

PROVIDER: S-EPMC10153730 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature