Elucidating dynamic anaerobe metabolism with HRMAS ¹³C NMR and genome-scale modeling.

ABSTRACT: Anaerobic microbial metabolism drives critical functions within global ecosystems, host-microbiota interactions, and industrial applications, yet remains ill-defined. Here we advance a versatile approach to elaborate cellular metabolism in obligate anaerobes using the pathogen Clostridioides difficile, an amino acid and carbohydrate-fermenting Clostridia. High-resolution magic angle spinning nuclear magnetic resonance (NMR) spectroscopy of C. difficile, grown with fermentable ¹³C substrates, informed dynamic flux balance analysis (dFBA) of the pathogen's genome-scale metabolism. Analyses identified dynamic recruitment of oxidative and supporting reductive pathways, with integration of high-flux amino acid and glycolytic metabolism at alanine's biosynthesis to support efficient e

SUBMITTER: Pavao A 

PROVIDER: S-EPMC10154198 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature