LncRNA XIST regulates breast cancer stem cells by activating proinflammatory IL-6/STAT3 signaling.

ABSTRACT: Aberrant expression of XIST, a long noncoding RNA (lncRNA) initiating X chromosome inactivation (XCI) in early embryogenesis, is a common feature of breast cancer (BC). However, the roles of post-XCI XIST in breast carcinogenesis remain elusive. Here we identify XIST as a key regulator of breast cancer stem cells (CSCs), which exhibit aldehyde dehydrogenase positive (ALDH⁺) epithelial- (E) and CD24^loCD44^hi mesenchymal-like (M) phenotypes. XIST is variably expressed across the spectrum of BC subtypes, and doxycycline (DOX)-inducible knockdown (KD) of XIST markedly inhibits spheroid/colony forming capacity, tumor growth and tumor-initiating potential. This phenotype is attributed to impaired E-CSC in luminal and E- and M-CSC activities in triple-negative (TN)

SUBMITTER: Ma Y 

PROVIDER: S-EPMC10154203 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature