Temperature sensitivity of carbon concentrating mechanisms in the diatom Phaeodactylum tricornutum.

ABSTRACT: Marine diatoms are key primary producers across diverse habitats in the global ocean. Diatoms rely on a biophysical carbon concentrating mechanism (CCM) to supply high concentrations of CO₂ around their carboxylating enzyme, RuBisCO. The necessity and energetic cost of the CCM are likely to be highly sensitive to temperature, as temperature impacts CO₂ concentration, diffusivity, and the kinetics of CCM components. Here, we used membrane inlet mass spectrometry (MIMS) and modeling to capture temperature regulation of the CCM in the diatom Phaeodactylum tricornutum (Pt). We found that enhanced carbon fixation rates by Pt at elevated temperatures were accompanied by increased CCM activity capable of maintaining RuBisCO close to CO₂ saturation but that the mec

SUBMITTER: Li M 

PROVIDER: S-EPMC10154264 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature