A rabies virus-based toolkit for efficient retrograde labeling and monosynaptic tracing.

ABSTRACT: Analyzing the structure and function of the brain's neural network is critical for identifying the working principles of the brain and the mechanisms of brain diseases. Recombinant rabies viral vectors allow for the retrograde labeling of projection neurons and cell type-specific trans-monosynaptic tracing, making these vectors powerful candidates for the dissection of synaptic inputs. Although several attenuated rabies viral vectors have been developed, their application in studies of functional networks is hindered by the long preparation cycle and low yield of these vectors. To overcome these limitations, we developed an improved production system for the rapid rescue and preparation of a high-titer CVS-N2c-ΔG virus. Our results showed that the new CVS-N2c-ΔG-based toolkit performed rem

SUBMITTER: Lin KZ 

PROVIDER: S-EPMC10154474 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature