Novel ¹³C enrichment technique reveals early turnover of DHA in peripheral tissues.

ABSTRACT: The brain is rich in DHA, which plays important roles in regulating neuronal function. Recently, using compound-specific isotope analysis that takes advantage of natural differences in carbon-13 content (¹³C/¹²C ratio or δ¹³C) of the food supply, we determined the brain DHA half-life. However, because of methodological limitations, we were unable to capture DHA turnover rates in peripheral tissues. In the current study, we applied compound-specific isotope analysis via high-precision GC combustion isotope ratio mass spectrometry to determine half-lives of brain, liver, and plasma DHA in mice following a dietary switch experiment. To model DHA tissue turnover rates in peripheral tissues, we added earlier time points within the diet switch study and took adva

SUBMITTER: Klievik BJ 

PROVIDER: S-EPMC10154972 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature