Optimization of a lipase/reduced graphene oxide/metal-organic framework electrode using a central composite design-response surface methodology approach.

ABSTRACT: Lipase has been gaining attention as the recognition element in electrochemical biosensors. Lipase immobilization is important to maintain its stability while providing excellent conductivity. In this study, a lipase electrochemical biosensor immobilized on a copper-centred metal-organic framework integrated with reduced graphene oxide (lipase/rGO/Cu-MOF) was synthesized by a facile method at room temperature. Response surface methodology (RSM) via central composite design (CCD) was used to optimize the synthesis parameters, which are rGO weight, ultrasonication time, and lipase concentration, to maximize the current response for the detection of p-nitrophenyl acetate (p-NPA). The results of the analysis of variance (ANOVA) showed that all three parameters were signifi

SUBMITTER: Mohd Mokhtar NAI 

PROVIDER: S-EPMC10155190 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature