Multimodal single-cell and whole-genome sequencing of small, frozen clinical specimens.

ABSTRACT: Single-cell genomics enables dissection of tumor heterogeneity and molecular underpinnings of drug response at an unprecedented resolution^1-11. However, broad clinical application of these methods remains challenging, due to several practical and preanalytical challenges that are incompatible with typical clinical care workflows, namely the need for relatively large, fresh tissue inputs. In the present study, we show that multimodal, single-nucleus (sn)RNA/T cell receptor (TCR) sequencing, spatial transcriptomics and whole-genome sequencing (WGS) are feasible from small, frozen tissues that approximate routinely collected clinical specimens (for example, core needle biopsies). Compared with data from sample-matched fresh tissue, we find a similar quality in the biological output

SUBMITTER: Wang Y 

PROVIDER: S-EPMC10155259 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature