Myeloid-like tumor hybrid cells in bone marrow promote progression of prostate cancer bone metastasis.

ABSTRACT:

Background

Bone metastasis is the leading cause of death in patients with prostate cancer (PCa) and currently has no effective treatment. Disseminated tumor cells in bone marrow often obtain new characteristics to cause therapy resistance and tumor recurrence. Thus, understanding the status of disseminated prostate cancer cells in bone marrow is crucial for developing a new treatment.

Methods

We analyzed the transcriptome of disseminated tumor cells from a single cell RNA-sequencing data of PCa bone metastases. We built a bone metastasis model through caudal artery injection of tumor cells, and sorted the tumor hybrid cells by flow cytometry. We performed multi-omics analysis, including transcriptomic, proteomic and phosphoproteomic analysis, to compare the difference betwee

SUBMITTER: Ye X 

PROVIDER: S-EPMC10155318 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature