ScRNASequest: an ecosystem of scRNA-seq analysis, visualization, and publishing.

ABSTRACT:

Background

Single-cell RNA sequencing is a state-of-the-art technology to understand gene expression in complex tissues. With the growing amount of data being generated, the standardization and automation of data analysis are critical to generating hypotheses and discovering biological insights.

Results

Here, we present scRNASequest, a semi-automated single-cell RNA-seq (scRNA-seq) data analysis workflow which allows (1) preprocessing from raw UMI count data, (2) harmonization by one or multiple methods, (3) reference-dataset-based cell type label transfer and embedding projection, (4) multi-sample, multi-condition single-cell level differential gene expression analysis, and (5) seamless integration with cellxgene VIP for visualization and with CellDepot for data hosting and

SUBMITTER: Li K 

PROVIDER: S-EPMC10155351 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature