Biosensor-guided rapid screening for improved recombinant protein secretion in Pichia pastoris.

ABSTRACT: Pichia pastoris (Komagataella phaffii) is widely used for industrial production of heterologous proteins due to high secretory capabilities but selection of highly productive engineered strains remains a limiting step. Despite availability of a comprehensive molecular toolbox for construct design and gene integration, there is high clonal variability among transformants due to frequent multi-copy and off-target random integration. Therefore, functional screening of several hundreds of transformant clones is essential to identify the best protein production strains. Screening methods are commonly based on deep-well plate cultures with analysis by immunoblotting or enzyme activity assays of post-induction samples, and each heterologous protein produced may require development of bespoke assa

SUBMITTER: Navone L 

PROVIDER: S-EPMC10155391 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature