Location and condition based reconstruction of colon cancer microbiome from human RNA sequencing data.

ABSTRACT:

Background

The association between microbes and cancer has been reported repeatedly; however, it is not clear if molecular tumour properties are connected to specific microbial colonisation patterns. This is due mainly to the current technical and analytical strategy limitations to characterise tumour-associated bacteria.

Methods

Here, we propose an approach to detect bacterial signals in human RNA sequencing data and associate them with the clinical and molecular properties of the tumours. The method was tested on public datasets from The Cancer Genome Atlas, and its accuracy was assessed on a new cohort of colorectal cancer patients.

Results

Our analysis shows that intratumoural microbiome composition is correlated with survival, anatomic location, microsatellite in

SUBMITTER: Sambruni G 

PROVIDER: S-EPMC10155404 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature