High-throughput UV-photofragmentation studies of thymine and guanine.

ABSTRACT: High-throughput photofragmentation studies of thymine and guanine were performed at 257 and 343 nm and for a wide range of ionisation laser intensities. Combining a continuous laser-based thermal desorption source with femtosecond multiphoton ionisation using a 50 kHz repetition rate laser allowed us to produce detailed 2D maps of fragmentation as a function of incident laser intensity. The fragmentation was distinctly soft, the parent ions being at least an order of magnitude more abundant than fragment ions. For thymine there was a single dominant fragmentation channel, which involves consecutive HNCO and CO losses. In contrast, for guanine there were several competing ones, the most probable channel corresponding to CH₂N₂ loss through opening of the pyrimidine ring

SUBMITTER: Wang S 

PROVIDER: S-EPMC10155487 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature