Tuning oxidative modification by a strong electric field using nanoESI of highly conductive solutions near the minimum flow rate.

ABSTRACT: Oxidative modification is usually used in mass spectrometry (MS) for labeling and structural analysis. Here we report a highly tunable oxidation that can be performed in line with the nanoESI-MS analysis at the same ESI emitter without the use of oxidative reagents such as ozone and H₂O₂, and UV activation. The method is based on the high-pressure nanoESI of a highly conductive (conductivity >3.8 S m^-1) aqueous solution near the minimum flow rate. The ion source is operated under super-atmospheric pressure (0.5 MPa gauge pressure) to avoid the contribution of electric discharge. The analyte at the tip of the Taylor cone or in the emitter droplet can be locally oxidized in an on-demand manner by varying the nanoflow rate. With an offline nanoESI, the degree

SUBMITTER: Han Z 

PROVIDER: S-EPMC10155921 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature