Quantifying cooperative multisite binding in the hub protein LC8 through Bayesian inference.

ABSTRACT: Multistep protein-protein interactions underlie most biological processes, but their characterization through methods such as isothermal titration calorimetry (ITC) is largely confined to simple models that provide little information on the intermediate, individual steps. In this study, we primarily examine the essential hub protein LC8, a small dimer that binds disordered regions of 100+ client proteins in two symmetrical grooves at the dimer interface. Mechanistic details of LC8 binding have remained elusive, hampered in part by ITC data analyses employing simple models that treat bivalent binding as a single event with a single binding affinity. We build on existing Bayesian ITC approaches to quantify thermodynamic parameters for multi-site binding interactions impacted by significant u

SUBMITTER: Estelle AB 

PROVIDER: S-EPMC10155966 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature