Transcriptional activation of the htrA (High-temperature requirement A) gene from Bartonella henselae.

ABSTRACT: Bacterial htrA genes are typically activated as part of the periplasmic stress response and are dependent on the extracytoplasmic sigma factor rpoE. A putative promoter region, P1, of the sigma(E)-type heat-inducible promoters has previously been identified upstream of the htrA gene of Bartonella henselae. Further analysis of the htrA mRNA by primer extension demonstrated that transcription initiates from P1 and a second region downstream of P1. This second promoter region, termed P2, had no sequence identity to sigma(E)-type heat-inducible promoters. Promoter regions were cloned individually and in tandem into pANT3 upstream of a promoterless version of the green fluorescent protein (GFP) gene (gfpmut3) and transformed into B. henselae by electroporation. The contiguous promoter region co

SUBMITTER: Resto-Ruiz SI 

PROVIDER: S-EPMC101561 | biostudies-literature | 2000 Oct

REPOSITORIES: biostudies-literature