Development of multifunctional synthetic nucleosomes to interrogate chromatin-mediated protein interactions.

ABSTRACT: Various proteins bind to chromatin to regulate DNA and its associated processes such as replication, transcription, and damage repair. The identification and characterization of these chromatin-associating proteins remain a challenge, as their interactions with chromatin often occur within the context of the local nucleosome or chromatin structure, which makes conventional peptide-based strategies unsuitable. Here, we developed a simple and robust protein labeling chemistry to prepare synthetic multifunctional nucleosomes that carry a photoreactive group, a biorthogonal handle, and a disulfide moiety to examine chromatin-protein interactions in a nucleosomal context. Using the prepared protein- and nucleosome-based photoaffinity probes, we examined a number of protein-protein and protein-n

SUBMITTER: Liu Z 

PROVIDER: S-EPMC10156118 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature