MRNA decoding in human is kinetically and structurally distinct from bacteria.

ABSTRACT: In all species, ribosomes synthesize proteins by faithfully decoding messenger RNA (mRNA) nucleotide sequences using aminoacyl-tRNA substrates. Current knowledge of the decoding mechanism derives principally from studies on bacterial systems¹. Although key features are conserved across evolution², eukaryotes achieve higher-fidelity mRNA decoding than bacteria³. In human, changes in decoding fidelity are linked to ageing and disease and represent a potential point of therapeutic intervention in both viral and cancer treatment^4-6. Here we combine single-molecule imaging and cryogenic electron microscopy methods to examine the molecular basis of human ribosome fidelity to reveal that the decoding mechanism is both kinetically and structurally distin

SUBMITTER: Holm M 

PROVIDER: S-EPMC10156603 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature