Ten-kilohertz two-photon microscopy imaging of single-cell dendritic activity and hemodynamics in vivo.

ABSTRACT:

Significance

The studying of rapid neuronal signaling across large spatial scales in intact, living brains requires both high temporal resolution and versatility of the measurement device.

Aim

We introduce a high-speed two-photon microscope based on a custom-built acousto-optic deflector (AOD). This microscope has a maximum line scan frequency of 400 kHz and a maximum frame rate of 10,000 frames per second (fps) at 250×40  pixels . For stepwise magnification from population view to subcellular view with high spatial and temporal resolution, we combined the AOD with resonance-galvo (RS) scanning.

Approach

With this combinatorial device that supports both large-view navigation and small-view high-speed imaging, we measured dendritic calcium propagation velocity and the

SUBMITTER: Li R 

PROVIDER: S-EPMC10156610 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature