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Cell-cell adhesions in embryonic stem cells regulate the stability and transcriptional activity of β-catenin.

ABSTRACT: E-cadherin (CDH1) is involved in maintaining cell-cell adhesions in embryonic stem cells (ESCs). However, its function in the context of cell fate decisions is largely unknown. Using mouse ESCs (mESCs), we demonstrate that E-cadherin and β-catenin interact at the membrane and continue to do so upon internalization within the cell. Cdh1-/- mESCs failed to form tight colonies, with altered differentiation, marker expression and retention of pluripotency factors during differentiation. Interestingly, Cdh1-/- mESCs showed dramatically reduced β-catenin levels. Transcriptional profiling of Cdh1-/- mESCs displayed a significant alteration in the expression of a subset of β-catenin targets in a cell state- and GSK3β-dependent manner. Our findings hint at hitherto unknown roles played by E-cadherin in regulating the activity of β-catenin in ESCs.

SUBMITTER: Bhattacharyya S 

PROVIDER: S-EPMC10156795 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature

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Cell-cell adhesions in embryonic stem cells regulate the stability and transcriptional activity of β-catenin.

Bhattacharyya Sinjini S   Mote Ridim D RD   Freimer Jacob W JW   Tiwari Mahak M   Bansi Singh Surya S   Arumugam Sandhya S   Narayana Yadavalli V YV   Rajan Raghav R   Subramanyam Deepa D  

FEBS letters 20220406 13

E-cadherin (CDH1) is involved in maintaining cell-cell adhesions in embryonic stem cells (ESCs). However, its function in the context of cell fate decisions is largely unknown. Using mouse ESCs (mESCs), we demonstrate that E-cadherin and β-catenin interact at the membrane and continue to do so upon internalization within the cell. Cdh1<sup>-/-</sup> mESCs failed to form tight colonies, with altered differentiation, marker expression and retention of pluripotency factors during differentiation. I  ...[more]

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