Mitochondrial-derived vesicles retain membrane potential and contain a functional ATP synthase.

ABSTRACT: Vesicular transport is a means of communication. While cells can communicate with each other via secretion of extracellular vesicles, less is known regarding organelle-to organelle communication, particularly in the case of mitochondria. Mitochondria are responsible for the production of energy and for essential metabolic pathways in the cell, as well as fundamental processes such as apoptosis and aging. Here, we show that functional mitochondria isolated from Saccharomyces cerevisiae release vesicles, independent of the fission machinery. We isolate these mitochondrial-derived vesicles (MDVs) and find that they are relatively uniform in size, of about 100 nm, and carry selective protein cargo enriched for ATP synthase subunits. Remarkably, we further find that these MDVs harbor a function

SUBMITTER: Hazan Ben-Menachem R 

PROVIDER: S-EPMC10157309 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature