The calcium-sensing receptor modulates the prostaglandin E₂ pathway in intestinal inflammation.

ABSTRACT: Introduction: The prostaglandin E₂ (PGE₂) pathway is one of the main mediators of intestinal inflammation. As activation of the calcium-sensing receptor (CaSR) induces expression of inflammatory markers in the colon, we assessed the impact of the CaSR on the PGE₂ pathway regulation in colon cancer cells and the colon in vitro and in vivo. Methods and Results: We treated CaSR-transfected HT29 and Caco-2 colon cancer cell lines with different orthosteric ligands or modulators of the CaSR and measured gene expression and PGE₂ levels. In CaSR-transfected HT29^CaSR-GFP and Caco-2^CaSR-GFP cells, the orthosteric CaSR ligand spermine and the positive allosteric CaSR modulator NPS R-568 both induced

SUBMITTER: Gushchina V 

PROVIDER: S-EPMC10157649 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature