Clickable Shiga Toxin B Subunit for Drug Delivery in Cancer Therapy.

ABSTRACT: In recent years, receptor-mediated drug delivery has gained major attention in the treatment of cancer. The pathogen-derived Shiga Toxin B subunit (STxB) can be used as a carrier that detects the tumor-associated glycosphingolipid globotriaosylceramide (Gb3) receptors. While drug conjugation via lysine or cysteine offers random drug attachment to carriers, click chemistry has the potential to improve the engineering of delivery systems as the site specificity can eliminate interference with the active binding site of tumor ligands. We present the production of recombinant STxB in its wild-type (STxB_wt) version or incorporating the noncanonical amino acid azido lysine (STxB_AzK). The STxB_wt and STxB_AzK were manufactured using a growth-decoupled

SUBMITTER: Danielewicz N 

PROVIDER: S-EPMC10157870 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature