Morphological, biological, and genomic characterization of Klebsiella pneumoniae phage vB_Kpn_ZC2.

ABSTRACT:

Background

Bacteriophages (phages) are one of the most promising alternatives to traditional antibiotic therapies, especially against multidrug-resistant bacteria. Klebsiella pneumoniae is considered to be an opportunistic pathogen that can cause life-threatening infections. Thus, this study aims at the characterization of a novel isolated phage vB_Kpn_ZC2 (ZCKP2, for short).

Methods

The phage ZCKP2 was isolated from sewage water by using the clinical isolate KP/08 as a host strain. The isolated bacteriophage was purified and amplified, followed by testing of its molecular weight using Pulse-Field Gel Electrophoresis (PFGE), transmission electron microscopy, antibacterial activity against a panel of other Klebsiella pneumoniae hosts, stability studies, and whole genome seque

SUBMITTER: Fayez MS 

PROVIDER: S-EPMC10158348 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature