Differentiated cultures of an immortalized human neural progenitor cell line do not replicate prions despite PrP^C overexpression.

ABSTRACT: Prions are misfolded proteins that accumulate within the brain in association with a rare group of fatal and infectious neurological disorders in humans and animals. A current challenge to research is a lack of in vitro model systems that are compatible with a wide range of prion strains, reproduce prion toxicity, and are amenable to genetic manipulations. In an attempt to address this need, here we produced stable cell lines that overexpress different versions of PrP^C through lentiviral transduction of immortalized human neural progenitor cells (ReN VM). Differentiated cultures made from the neural progenitor cell lines overexpressed PrP^C within 3D spheroid-like structures of TUBB3⁺ neurons and we observed evidence that PrP^C modula

SUBMITTER: Slota JA 

PROVIDER: S-EPMC10158546 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature