Photoactivatable surfaces resolve the impact of gravity vector on collective cell migratory characteristics.

ABSTRACT: Despite considerable interest in the impact of space travel on human health, the influence of the gravity vector on collective cell migration remains unclear. This is primarily because of the difficulty in inducing collective migration, where cell clusters appear in an inverted position against gravity, without cellular damage. In this study, photoactivatable surfaces were used to overcome this challenge. Photoactivatable surfaces enable the formation of geometry-controlled cellular clusters and the remote induction of cellular migration via photoirradiation, thereby maintaining the cells in the inverted position. Substrate inversion preserved the circularity of cellular clusters compared to cells in the normal upright position, with less leader cell appearance. Furthermore, the inversion

SUBMITTER: Sakakibara S 

PROVIDER: S-EPMC10158565 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature