Design of cell expansion processes for adherent-growing cells with mDoE-workflow.

ABSTRACT: Adherent cells, mammalian or human, are ubiquitous for production of viral vaccines, in gene therapy and in immuno-oncology. The development of a cell-expansion process with adherent cells is challenging as scale-up requires the expansion of the cell culture surface. Microcarrier (MC)-based cultures are still predominate. However, the development of MC processes from scratch possesses particular challenges due to their complexity. A novel approach for the reduction of development times and costs of cell propagation processes is the combination of mathematical process models with statistical optimization methods, called model-assisted Design of Experiments (mDoE). In this study, an mDoE workflow was evaluated successfully for the design of a MC-based expansion process of adherent L929 cells

SUBMITTER: Kuchemuller KB 

PROVIDER: S-EPMC10158623 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature