Comparison of the structures and topologies of plasma extracted circulating nuclear and mitochondrial cell-free DNA.

ABSTRACT: Introduction: The function, origin and structural features of circulating nuclear DNA (cir-nDNA) and mitochondrial DNA (cir-mtDNA) are poorly known, even though they have been investigated in numerous clinical studies, and are involved in a number of routine clinical applications. Based on our previous report disproving the conventional plasma isolation used for cirDNA analysis, this work enables a direct topological comparison of the circulating structures associated with nuclear DNA and mitochondrial cell-free DNA. Materials and methods: We used a Q-PCR and low-pass whole genome sequencing (LP-WGS) combination approach of cir-nDNA and cir-mtDNA, extracted using a procedure that eliminates platelet activation during the plasma isolation process to prevent mitochondria releas

SUBMITTER: Pisareva E 

PROVIDER: S-EPMC10158822 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature