System metabolic engineering of Escherichia coli W for the production of 2-ketoisovalerate using unconventional feedstock.

ABSTRACT: Replacing traditional substrates in industrial bioprocesses to advance the sustainable production of chemicals is an urgent need in the context of the circular economy. However, since the limited degradability of non-conventional carbon sources often returns lower yields, effective exploitation of such substrates requires a multi-layer optimization which includes not only the provision of a suitable feedstock but the use of highly robust and metabolically versatile microbial biocatalysts. We tackled this challenge by means of systems metabolic engineering and validated Escherichia coli W as a promising cell factory for the production of the key building block chemical 2-ketoisovalerate (2-KIV) using whey as carbon source, a widely available and low-cost agro-industrial waste. First,

SUBMITTER: Carranza-Saavedra D 

PROVIDER: S-EPMC10158823 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature