Allosteric regulation of mammalian Na⁺/I^- symporter activity by perchlorate.

ABSTRACT: The Na⁺/I^- symporter (NIS), the plasma membrane protein that actively transports I^- (stoichiometry 2Na⁺:1I^-) in thyroid physiology and radioiodide-based thyroid cancer treatment, also transports the environmental pollutant perchlorate (stoichiometry 1Na⁺:1ClO₄^-), which competes with I^- for transport. Until now, the mechanism by which NIS transports different anion substrates with different stoichiometries has remained unelucidated. We carried out transport measurements and analyzed these using a statistical thermodynamics-based equation and electrophysiological experiments to show that the different stoichiometry of ClO₄^- transport is due to ClO₄^- b

SUBMITTER: Llorente-Esteban A 

PROVIDER: S-EPMC10158964 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature